Fig. 6
From: Multiple genetically engineered humanized microenvironments in a single mouse
Analysis of soluble microenvironments dependent hCD34 cell behavior after co-implantation with hBMSCs and genetically engineered mBMSCs. a Schematic of experimental procedure. Once hBMSCs and growth-arrested genetically engineered mBMSCs formed stable adhesion on pore surface, the pore space was filled with hCD34 and HUVECs dispersed in liquid state ice-cold matrigel. Matrigel was subsequently solidified and hCD34 cell-retaining scaffolds were implanted to NSG mice for 3 weeks. b Immunohistostaining of explanted scaffolds post 3 weeks implantation. (Green: mCD31, red: hCD34, and blue: nucleus/Scale bar, 200 μm). c Representative images of colony forming units after 14 days methylcellulose plating (Scale bar, 300 μm), d Compression of total CFU counting in different stromal-cell implants. e-f Comparison of specific CFU counting for differentiated hematopoietic cell types; (e) CFU-Erythroid (CFU-E), (f) Burst Forming Unit-Erythroid (BFU-E), (g) CFU-Granulocyte and Macrophage (CFU-GM), (h) CFU-Granulocyte, Erythrocyte, Monocyte, and Megakaryocyte (CFU-GEMM). (N = 3, *P < 0.5, **P < 0.05)